Readers ask: What Did The Manual Manipulation (smashing) Of The Fruit Accomplish?


What does mashing do to the fruit in DNA extraction?

Mashing the banana exposes a greater surface area from which to extract the DNA. The liquid soap is added to help break down cell membranes to release the DNA. The filtration step (pouring the mixture through the strainer) allows for the collection of the DNA and other cellular substances.

Why do you smash strawberries in DNA extraction?

The physical smashing breaks the plant’s cell walls and allows the cytoplasm to leak out. Add 10mL (2 teaspoons) of extraction buffer (salt and soap solution) to the bag. Kneed/mush the strawberry in the bag again for 1 minute.

What is the purpose of crushing the strawberry in extraction solution What does this do to the strawberry’s cells and DNA?

In this procedure, you will crush a strawberry and add detergent and salt to break down the cell walls to release the DNA within the nucleus. You will then filter the liquid from this crushed strawberry into a beaker, the substance is called the filtrate.

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Why is it important to break down membranes inside the cell?

Breaking the nuclear membrane exposes the DNA to reactive and possibly dangerous chemicals in the cell. To slow down these chemical reactions, which might damage or chop up the DNA, students use cold ingredients and add cold alcohol.

What is the best fruit to extract DNA from?

Experiment to purify DNA from fruit Bananas, kiwis and strawberries all work well. (Remove the skin of the bananas and kiwi, we just want the insides!) Step 2: In a separate bowl, mix the washing up liquid, salt and tap water.

What is the purpose of extracting DNA from a banana?

Explain that crushing the bananas separates its cells and exposes them to the soap and salt. The soap helps break down cell membranes and release DNA. The salt helps bring the DNA together, and the cold alcohol helps the DNA precipitate and come out of solution so it can be collected.

Does human DNA look like strawberry DNA?

Each little piece of a living thing, known as a cell, has DNA in it. In humans each of these cells have 2 copies of the DNA, but in strawberries each of these have 8 copies of the DNA (scientists call this octoploid). That means strawberries have 4 times as many copies of DNA as humans, making it 4 times easier to see!

Why do uneaten strawberries eventually rot?

All three can result from excessive moisture and warm, humid weather. Because strawberry plants are low, it is easy for water to splash dirt on them and contaminate them, and for them to get wet and stay wet. As a last resort, or if mold and rot continue to pester your plants, you can use a fungicide.

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Why does salt make DNA clump together?

DNA is a double helix with negatively charged phosphate groups in the backbone. The salt neutralizes these charges and lets DNA strands clump together when isopropyl alcohol is added. In solution, these strands have a slight negative electric charge.

Can you eat strawberry DNA?

You can extract DNA from fresh strawberries. I wouldn’t eat the DNA alone though. When wet it is slimy and when dry it looks like cotton wool. But when mixed with the other components of strawberries it is undetectable and harmless, and strawberries taste great as they are.

What cells would you use to extract DNA from a living person?

If you wanted to extract DNA from a living person, which cells would you use and why? Epithelial cells will work if you need only a small amount of DNA. However, blood is the easiest tissue to obtain from a living person. Although a red blood cell does not contain a nucleus, a white blood cell does.

What does DNA contain the instructions for?

DNA contains the instructions needed for an organism to develop, survive and reproduce. To carry out these functions, DNA sequences must be converted into messages that can be used to produce proteins, which are the complex molecules that do most of the work in our bodies.

What happens to membrane permeability below 0?

Generally, increasing the temperature increases membrane permeability. At temperatures below 0 o C the phospholipids in the membrane don’t have much energy and so they can’t move much, which means that they’re closely packed together and the membrane is rigid.

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What effect did the detergent have on cell membranes?

Membrane disruption, protein binding and solubilization Generally, moderate concentrations of mild (i.e., nonionic) detergents compromise the integrity of cell membranes, thereby facilitating lysis of cells and extraction of soluble protein, often in native form.

What is the head of a phospholipid made of?

The phospholipids have a hydrophilic (water attracting) HEADS and two hydrophobic (water repelling) TAILS. The head of a phospholipid is made of an alcohol and GLYCEROL group, while the tails are chains of FATTY ACIDS.

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